Methodology
1.
Bacterioscophy
2. Test on
movement on 0,3 % semi-liquid agar by punching
3. Test of
pathogens on 5% defibrinated blood mixed agar
4. Biochemical
testing
5.
Serodiagnostics with E-coli polyvalent strain O-H antigen
6. Testing of
sensitivity on the preparations of bacteriophages by spot-test
method
Research
We have taken
from Patardzleuli farm: fecal wastes, pathogenic organs, heart, liver,
bladder and
rectum of 6
day old dead chickens. (See table-1).
We took samples of water and food.( See
table 2 (See
table 3)
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E-coli
sensitivity on Pio
and
Intesti bacteriophages |
Antibioticograma
|
E-coli
on endo differentiating
soil |
Enterococcus colonies
after 48
houresof
incubation |
The materials
were seeded in the sugar bullion. Other organs were cleaned with
disinfecting liquid,
where had been
done cuts and the samples were taken from inside layers. The research
was provided by bacteriological, and serological methodology. For this
purposes we have selected elective-selective soils, used
differentiating- diagnostic endo soils, Plaskirev, Mac-Kinski,
Ecterococcus and Pseudomonas agars. Has been studied bacterioscopy of
separate isolates. We used Gram’s method of painting. Isolated isolate
was seeded one semi-mixture 0,3% agare for the purpose of studying the
movement by pricking. As well as we used hanging spot’s method for the
identification of Salmonella gallenarum-pullorum.
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Ms.N,Makhadze and Ms.N.Japarashvili in the
laboratory of JSC Biochimfarm |
Dr. Merab Natidze in the
laboratory of JSC Biochimfarm |
Ms.N.Japarashvili
and Dr. Merab Natidzein
the laboratory of JSC Biochimfarm |
Ms.N.Japarashvili,
Ms.E.Mamuchishvili and
Ms.Patarkatsishvili in Patardzleuli Farm |
Has been
provided serodiagnostics of ABCD adsorbed serum Selected pathogenic
strains was tested on sensitivity toward 12 antibiotics. Antibiotic
resistant strains has undergone phage diagnostics by existing
intesti and pio bacteriophages. The strains which were
resistant to phages were seeded into the water of farm #1 where has
been separated E-coli and Salmonella phages. Has been
conducted several passages (for the purpose of increasing activity)
The selected phages were bottled and given to farm #1 (please see
table 1, 2)
We provide
testing of influence of intestibacteriophages for preventive purposes
on the organisms of
birds in farms
(#1, #2, #3) Our team provided identification of keeping status of
ntestibacteriophages
by means of
pH tests in crop, stomach and intestines of birds.
The
abovementioned testing was conducted due to optimal nature of phages
action. We made parallel tests on intestibacteriophages. pH 5,0 (acid
environment) and pH 8,0 practically make no influence on the activity
of phages during 24 hours. During research the research of digestive
system we identified, that pH in 10-28 day chickens fluctuates from 8
to 5,5, in stomach 3,0-4,0 and in intestines 6,0.
For the
purpose of identification of the efficiency of bacteriophages
preparations the 1 day chickens of Patardzleuli farm has been given
preparations of intestibacteriophages during 5 days. The doze of
2,106
has been given to 1100 wings during five days (Experimental
goup) The other 1 100 wings has
been treated
by polyvitamine and antibiotics (Traditional theraphy group). In
experimental group 99,8
% of chickens
escaped. In traditional therapy group 99,1%.
The the daily
growth in weight constituted: 4,5 grams ( experimental group) and 3,8
(traditional
theraphy
group)
In Krtsanisi
farm 1 day chickens has been given preparations of
intestibacteriophages during 7 days.
The same doze
has been given to 1100 wings during five days (Experimental goup) The
other 1 400
wings has been
treated by polyvitamine and antibiotics (Traditional therapy group).
In experimental
and
traditional therapy groups 99,8% chickens escaped.
The the daily
growth in weight constituted: 4,8 grams ( experimental group) and 4
(traditional therapy group)
